Download PDF by Pill-Soon Song: Annual European Symposium on Photomorphogenesis.

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By Pill-Soon Song

ISBN-10: 0080226779

ISBN-13: 9780080226774

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Extra info for Annual European Symposium on Photomorphogenesis. Photochemistry and Photobiology

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The homogenate was strained through six layers of cheese cloth and immediately subjected to a short time centrifugation by bringing up the centrifuge (Christ Zeta 20) to maximum speed (about 9000 x g) and then switching it off. 01 M MgCl and 3% 2 Triton X-100. After shaking the mixture for 10 min at 2°C it was subjected to another short time centrifugation (see above) with a maximum speed of about 2500 x g. While the pellet formed consisted mainly of nuclei the chloroplast material was recovered in the supernatant from which the nucleic acids were subsequently extracted.

Much to our surprise the spectral dependence for the second light flash was qualitatively different from that for the first light flash. The most conspicuous differences were the d r o p in activity at 650 n m a n d longer wavelengths and an increase in activity of green light. However, a minimum was found in approximately the same region 475-500 n m as for the first light flash. Also, the maxim u m in the blue region was found at the same wavelength, namely at 4 2 5 ^ 5 0 nm. Differential screening from other pigments might well influence the appearance of the spectral activity before and after the first flash of light.

A recent action spectrum for the induction of the response supports this conclusion for maize (Fuad and Yu, 1977). The term "in vivo binding" has been used to describe the pelletability that results exclusively from irradiations of intact tissue (Schafer et al 1975; Marme et al 1976; Pratt and Marme, 1976; Marme, 1977). However, while existing data permit the conclusion that there is a truly intracellular component in the overall process that leads to enhanced pelletability, they do n o t permit a decision as to whether this component includes the final binding step itself.

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Annual European Symposium on Photomorphogenesis. Photochemistry and Photobiology by Pill-Soon Song


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